Proteomics seminar series for the South East
---Registration is free---
-- The London Proteomics Discussion Group --
Proteomics seminar series for the South East
Proteomics seminar series for the South East
We are a free, local proteomics seminar series in the South East,
with a focus towards networking, discussion and supporting early career researchers.
The LPDG...
was founded to bring together the large community of proteomics scientists all working in and around London. We aim to provide a space for discussion, with a focus on methods and early career researchers (two fundamental building blocks of good research!), on all topics related to proteomics. The meetings comprise of research talks framed by a proteomics methods challenge, lunch, refreshments and pizza - they are free to attend thanks to sponsorship.
Meeting Dates:
These seminars would not be possible without our amazing sponsors.
If you are interested in sponsoring an LPDG seminar,
please get in touch at sponsor@londonproteomics.co.uk
06th August 2021 14:00 GMT
Expanding the subunit complexity of HDX/MS experiments: Using cyclic IMS to investigate the conformations of bovine mitochondrial complex I
Mitochondrial complex I (NADH:ubiquinone oxidoreductase), the largest protein assembly of the respiratory chain has a total mass of approximately 970 kilodaltons and drives cellular energy production by transferring electrons from NADH to ubiquinone with a concomitant translocation of protons across the inner mitochondrial membrane. The complex is made up of 45 individual subunits, 14 core subunits that contain the proteins involved in catalysis (and are conserved across species from bacteria to humans) and 31 supernumerary subunits that are specific to mammals. During single-particle cryo-electron microscopy studies of the enzyme by the group of Judy Hirst (MRC-Mitochondrial Biology Unit), computational sorting of the particles identified different structural classes, which showed slight conformational changes. Biochemically these movements have been described as the 'active-to-de-active' enzyme transition. I will discus the application of cyclic IMS to hydrogen deuterium exchange studies of complex I in the so called ‘active’ and ‘de-active’ conformations. The increased ion mobility resolution and ms resolution of the cyclic has allowed us to separate overlapping peptides so increasing our peptic peptide coverage of individual subunits and enabling us to increase the subunit complexity of the systems studied by HDX/MS.
Discovering the topology of protein complexes in situ using crosslinking-MS
Identifying the components and topologies of labile protein complexes in their native environments remains a major challenge in structural biology. Crosslinking mass spectrometry can identify residues that are nearby in space and is routinely used to study the structure of protein complexes in vitro. We have pushed this technology towards mapping the topologies of of protein complexes inside intact cells.
I will show that the powerful combination of in cell cross-linking mass spectrometry and cryo-electron microscopy can expand our understanding of even ‘well-characterised’ protein complexes. We demonstrate this by describing the architecture of a novel complex between a bacterial RNA polymerase and ribosome bridged through NusA, an anti-termination factor ubiquitous in bacteria.
Would you like to present at an LPDG meeting? Email: speaker@londonproteomics.co.uk
Research presentations from:
Having received a B.Sc (Hons) in Chemistry with Biochemistry from King’s College, London (1988), Mark took a position as Higher Scientific Officer in John E. Walker’s lab at the MRC LMB, Cambridge, applying protein sequencing techniques for subunit characterisation of bovine mitochondrial NADH:Ubiquinone Oxidoreductase. He was awarded a Ph.D. in 1994. In 1998 he joined SmithKline Beecham before returning to academic science in 2007, setting up a biological mass spectrometry lab at CRUK. In 2012 he returned to the LMB as Head of Biological MS and Proteomics before joining the Crick as Head of the Proteomics STP this year.
Francis O'Reilly studied at the University of Edinburgh where he obtained a Master's by Research in Structural Biology. Following this, he completed his Ph.D. studies at the European Molecular Biology Laboratory (EMBL) in Heidelberg where he developed methods to structurally study protein complexes in cell lysates. Currently, he is a postdoctoral fellow at the Technical University Berlin in Prof. Juri Rappsilber’s lab where he is using proteomics and electron microscopy to study the structure of protein complexes inside cells.
Dr. Hoopmann’s research is focused on proteomics technology and methods development, having been trained in both instrumentation and software data analysis, with particular focus on high-resolution mass spectrometry. His current interests are in the development of advanced algorithms for discovery-based proteomics. He is the developer of the Kojak algorithm, a versatile, open-source software application for the discovery of protein-protein interactions through shotgun-based mass spectrometry. Dr. Hoopmann is also a contributor to the Trans-Proteomic Pipeline suite of software solutions for mass spectrometry data analysis.
March 2020 Meeting
Here is a list of answers to frequently asked questions for speakers, delegates and sponsors
The meetings are free to attend! We raise money through the generous support of sponsors for refreshments and venue hire. Sponsors are offered the opporunity to suggest a plenary research presentaiton or present a short (10min) sales talk.
Yes! Thanks again to the support of our sponsors, we are able to provide a sandwhich buffet lunch, refreshments, and pizza and drinks to end. We try our best to get a range of refreshments to support diverse dietry requirments, but please let us know in advance if you have any specific needs.
We are always looking for new speakers - from PhD candidates to senior scientists within proteomics. We recruit speakers through personal invitations/networking, suggestions from deligates and from people contacting us directly. If you would like to present at a meeting, we would be delighted to hear from you, please get in touch at speaker@londonproteomics.co.uk
Registration is not essential, but it is very helpful for us to arrange catering and venues to ensure we don't over/under spend. In some cases, where space is limiting, registration may be essential and we will advertise this. If in doubt - register!
We do not have any funding to support delegates. However, if you are speaking, it may be possible to get a travel grant from the British Society for Proteome Research (BSPR). In some instances, we may be able to subsidise travel costs for speakers, but this is dependant on sponsorship for that meeting.
The organising committee is made up of early career (including PhD candidates) and more experienced scientist from academia and industry. We are very keen to have a bredth of backgrounds and experience on the committee, so if you would like to be involved, please get in touch by speaking to a committee member at a meeting, or emailing us - we would like to hear from you!
Our meetings are focused on proteomics and the delegates include proteomics-users from academia and industry. Therefore, at these meetings, there is a "captured audience" of possible clients. Sponsoring our meeting will allow you to present your product or service to a concentrated group of possible users. These meetings are only possible with sponsorship, and you will be contributing to the disemiation of science, and potentially meeting tallented future employees through networking.
We have a range of options for meeting sponsorship. To sponsor a meeting, please get in touch at sponsor@londonproteomics.co.uk, we will be delighted to hear from you.
Yes! Just like our physical meetings, our webinars are completely free to attend.
Please register for our meeting in the normal way (click here). A link will be emailed to you prior to the meeting starting with instructions on how to join. Alternatively, a join link for the webinar will also be posted here immediately prior to it starting. For webinars run as a Microsoft Live Event, find instructions on how to join here, or using Zoom here.
The discussion will be mediated by the chairperson who will take questions from all attendies. The best way to contribute during the talk and to post questions is through our slack account.
The meetings are recored and will be posted to our archive page.
The webinars are put on with the generous support academic institutions (as indicated in the webinar acknowledgements). We may accept sponsorship for webinars to allow us to run competitions and maintain our ability to put on free meetings in high quality venues. Webinar sponsors get the opportunity of a short presentation at the beginning of the webinar, and we may disseminate some information through our mailing list after the event. Sponsorship does not represent endorsement by LPDG or its affiliates.
If you still have unanswered questions after reading this page, wish to present a talk, suggest a venue or sponsor a meeting, please contact us.
The organising committee is made up of early and "not-so-early" career scientists
from academia and industry.
If you are interested in joining the committee, please get in touch.
After my PhD in blood plasma cancer proteomics I moved to the Cancer Proteomics Group at UCL. I founded the LPDG as a focus group for the SE. I am currently at the Babraham Institute investigating protein degradation pathways using proteomics.
I am a post doc at ICL, developing mass spectrometry and data analysis methodology for the study of protein PTMs, in particular methylation. My research is multidisciplinary, using chemistry, bioinformatics and biology. For more info, click here.
I am a post-doc at the ICR in Paul Huang’s group. My research interest is in extracellular matrix remodelling during cancer progression. From an analytical point of view, I am interested in protein quantification by DIA mass spectrometry.
I manage an MS lab at QMUL. I graduated from Drexel University (USA) in Biomedical Science, completing my PhD work at Harvard University. After a long stint in the private sector, I re-joined academia here in the UK researching lipidomics and proteomics.
Danai studied biology and biomedical sciences in Greece. She has worked in numerous labs in England, Singapore, the Netherlands and Greece. Now she is focusing on her PhD at UCL in Primary Biliary Cholangitis analyzing human samples using Mass spectrometry.
I am a PhD student at UCL on the CellX project in the Thalassinos Lab studying competition in cellular populations using mass spectrometry-based proteomics.
Daniel is a post-doc in the lab of Prof. Ed Tate at Imperial College London. His research interests lie in the use of chemical proteomics for better understanding of drug targets, protein function, and post-translational modification dynamics.
Tom works for SCIEX in the London region helping customers with MS and 'OMICS applications. He gained an interest in MS from working with Prof Roy Goodacre, Manchester Institute of Biotech. applying -omics and chemometric approaches for rapid food authenticity determination.
Please email with any questions.
Particularly welcome are venue suggestions,
speaker suggestions or if you are thinking of sponsoring a meeting.
This seminar series is run by volunteers from academia and industry. We will try to reply to your email as quickly as possible, but please allow at least 5 days.
