LPDG

-- The London Proteomics Discussion Group --
Proteomics seminar series for the South East

Next Webinar LPDG-YPIC Mini Challenge
Proteomics seminar series for the South East
Discussion
Networking
Methods Challenge
---Registration is free---

About the LPDG

We are a free, local proteomics seminar series in the South East,
with a focus towards networking, discussion and supporting early career researchers.

The LPDG...

was founded to bring together the large community of proteomics scientists all working in and around London. We aim to provide a space for discussion, with a focus on methods and early career researchers (two fundamental building blocks of good research!), on all topics related to proteomics. The meetings comprise of research talks framed by a proteomics methods challenge, lunch, refreshments and pizza - they are free to attend thanks to sponsorship.


Meeting Dates:

Next Meeting

Sponsors

These seminars would not be possible without our amazing sponsors.
If you are interested in sponsoring an LPDG seminar,
please get in touch at sponsor@londonproteomics.co.uk

LPDG-YPIC Mini Challenge

The LPDG have teamed up with YPIC to produce a proteomics-challenge with Prize money sponsored by Matrix Science. To find out more and view the current challenge,

click here!

Webinar Programme


Proteomics: The Role Of Chemical Proteomics

18th June 2021 14:00 GMT

Dr Ilaria Piazza

How Proteins and Metabolites Handshake: proteomics technologies for studying protein-small molecule intersactions

Different environmental cues such as stress, nutrients or drugs, trigger rapid adaptive responses that allow to maintain cellular homeostasis. One of the fastest cellular responses to the environment is the binding of small molecules to proteins. These molecular interactions produce allosteric effects, which means that they trigger a variation of protein activity as a consequence of a conformational change. Allosteric interactions are thus essential for life and can modulate both the metabolic status of the cells and gene expression. My group focuses on the development of new MS-bases proteomics technologies to study global structural changes in the proteome. In my talk I will describe the applications of a proteomic technique called LiP-MS to discover novel protein-metabolite interactions in bacteria and its more recent applications for the study of the protein small-molecule interactome in human cells.

Publication

Patrick Zanon

Profiling the Proteome-Wide Selectivity of Diverse Electrophiles

Targeted covalent inhibitors (TCIs) have experienced a resurgence of interest for drug discovery applications as they can have key advantages such as increased binding affinity to the target and the possibility to generate selectivity among closely related proteins. Competitive residue-specific proteomics allows the streamlined identification of on- and off-targets of TCIs and thus aids the development of selective ligands. Since most TCIs so far address cysteine residues because of their inherent outstanding nucleophilicity, the number of potentially addressable proteins is significantly limited by the requirement for these rare reactive cysteines. While many electrophilic groups have been reported to engage other amino acids, their selectivity towards the proteome has hardly ever been assessed and differences in the experimental workflow and the data analysis make it impossible to directly compare different studies. To address these issues, we combined our recently developed isoDTB-ABPP platform with the FragPipe software suite into a universal and unbiased workflow that identifies the modification caused by a covalent alkyne probe and the sites it occurs on in a quantitative fashion. We analysed >50 such electrophiles and verified or newly identified probes to selectively monitor nine different amino acids and the N-terminus proteome-wide. This toolset will enable competitive profiling of TCIs against a variety of reactive amino acids and thereby guide the development of novel TCIs.

Publication

Event Speakers

Would you like to present at an LPDG meeting? Email: speaker@londonproteomics.co.uk
Research presentations from:

Patrick Zanon
Patrick Zanon
Patrick Zanon Technical University of Munich

Patrick Zanon has obtained his B.Sc. and M.Sc. in chemistry at the Technical University of Munich, where he is currently pursuing a PhD in the group of Dr. Stephan M. Hacker. Funded by a Liebig PhD fellowship by the Fonds der Chemischen Industrie, he developed the isoDTB-ABPP platform, which allows the residue-specific identification of the target proteins of covalent ligands. By applying this method to so far underexplored nucleophilic amino acids, he aims to expand the fraction of the proteome that is deemed “druggable”. This research is motivated by the need to identify lead compounds with new modes-of-action to combat antibiotic resistance.

Dr Ilaria Piazza
Dr Ilaria Piazza
Dr Ilaria Piazza Max Delbruck Center of Molecular Medicine
Berlin

My research started with my PhD studies at EMBL in Heidelberg, studying molecular machines regulating chromatin structure and gene expression during the cell cycle. After stumbling into crosslinking MS in the Beck laboratory, I studied protein structures in the Picotti laboratory at ETH Zurich. I developed a method that combines limited proteolysis and MS to characterise interactions between proteins and metabolites (LiP-SMap). The goal of my research as group leader is to study how metabolism regulates chromatin architecture and its impact on gene expression.

Dr Marcus Bantscheff
Dr Marcus Bantscheff
Dr Marcus Bantscheff
Guest Chair

Coming soon!

Meetings
Total
Speakers
Total
Attendees
YouTube
views

registration

FAQ

Here is a list of answers to frequently asked questions for speakers, delegates and sponsors

If you still have unanswered questions after reading this page, wish to present a talk, suggest a venue or sponsor a meeting, please contact us.

Organising Committee

The organising committee is made up of early and "not-so-early" career scientists
from academia and industry.
If you are interested in joining the committee, please get in touch.

Dr Harvey Johnston
Dr Harvey Johnston Chairperson, Founder

After my PhD in blood plasma cancer proteomics I moved to the Cancer Proteomics Group at UCL. I founded the LPDG as a focus group for the SE. I am currently at the Babraham Institute investigating protein degradation pathways using proteomics.

Dr Harry Whitwell
Dr Harry Whitwell Communications Officer

I am a post doc at ICL, developing mass spectrometry and data analysis methodology for the study of protein PTMs, in particular methylation. My research is multidisciplinary, using chemistry, bioinformatics and biology. For more info, click here.

Dr Lukas Krasny
Dr Lukas Krasny Secretary

I am a post-doc at the ICR in Paul Huang’s group. My research interest is in extracellular matrix remodelling during cancer progression. From an analytical point of view, I am interested in protein quantification by DIA mass spectrometry.

Dr Roberto Buccafusca
Dr Roberto Buccafusca Treasurer

I manage an MS lab at QMUL. I graduated from Drexel University (USA) in Biomedical Science, completing my PhD work at Harvard University. After a long stint in the private sector, I re-joined academia here in the UK researching lipidomics and proteomics.

Danai Kati
Danai Kati
Danai Kati Committee Member

Danai studied biology and biomedical sciences in Greece. She has worked in numerous labs in England, Singapore, the Netherlands and Greece. Now she is focusing on her PhD at UCL in Primary Biliary Cholangitis analyzing human samples using Mass spectrometry.

Suniya Khatun
Suniya Khatun Committee member

I am a PhD student at UCL on the CellX project in the Thalassinos Lab studying competition in cellular populations using mass spectrometry-based proteomics.

Dr Daniel Conole
Dr Daniel Conole Committee member

Daniel is a post-doc in the lab of Prof. Ed Tate at Imperial College London. His research interests lie in the use of chemical proteomics for better understanding of drug targets, protein function, and post-translational modification dynamics.

Tom Ruane
Tom Ruane Committee member

Tom works for SCIEX in the London region helping customers with MS and 'OMICS applications. He gained an interest in MS from working with Prof Roy Goodacre, Manchester Institute of Biotech. applying -omics and chemometric approaches for rapid food authenticity determination.

Joanna Kirkpatrick
Dr Joanna Kirkpatrick
Dr Joanna Kirkpatrick Committee member

Joanna Kirkpatrick
Crick Insitute

London and the South East
United Kindgom

Please email with any questions.
Particularly welcome are venue suggestions,
speaker suggestions or if you are thinking of sponsoring a meeting.

This seminar series is run by volunteers from academia and industry. We will try to reply to your email as quickly as possible, but please allow at least 5 days.